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In tempo reale immagini di leucotriene B 4 Migrazione cellulare mediata e BLT1 Interazioni con β-arrestina
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13:45 min
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December 23, 2010
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Questo documento descrive la metodologia per determinare la risposta chemiotattica dei leucociti ai ligandi specifici e identificare le interazioni tra recettori di superficie della cellula e le proteine citosoliche utilizzando vivere tecniche di imaging cellulare.
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Leukotriene B4Cell MigrationBLT1β-arrestinG-protein Coupled ReceptorsTransmembrane Protein FamilyExtracellular SignalsIntracellular ResponsesChemotaxisCalcium ReleaseGene RegulationLigand BindingConformational ChangesHeterotrimeric G-proteinsSecond MessengersCyclic Adenosine Monophosphate (cAMP)Inositol Triphosphate (IP3)Diacyl Glycerol (DG)DesensitizationSequestrationInternalizationG-protein Receptor Kinases (GRKs)Cytosolic ProteinsMembrane TranslocationPro-inflammatory Lipid MoleculeArachidonic Acid Pathway
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Jala, V. R., Haribabu, B. Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin. J. Vis. Exp. (46), e2315, doi:10.3791/2315 (2010).
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