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从啮齿动物的神经元和非人类灵长类动物的脑片DiOLISTIC标签
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
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从啮齿动物的神经元和非人类灵长类动物的脑片DiOLISTIC标签

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09:21 min

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July 06, 2010

DOI:

10.3791/2081-v

DOI:
10.3791/2081-v

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09:21 min
July 06, 2010

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3 Views
, , , ,
1Section on Neuronal Structure, Laboratory for Integrative Neuroscience, NIAAA,NIH, 2Department Physiology and Pharmacology,Wake Forest University Health Sciences, 3Oregon National Primate Research Center, Division of Neuroscience,Oregon Health and Science University

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DiOLISTIC LabelingNeuronsRodent Brain SlicesNon-human Primate Brain SlicesGene GunFluorescent DyesDiIBrain Slice StainingTechnique SetupDiI-coated BulletsFixative ExposureDetergent ConcentrationCommon ProblemsVersatile Labeling TechniqueAnimal SpeciesAge RangeSpecies And Genotype FlexibilityImmunostainingDendrite BranchingDendritic Spine Morphology

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Seabold, G. K., Daunais, J. B., Rau, A., Grant, K. A., Alvarez, V. A. DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices. J. Vis. Exp. (41), e2081, doi:10.3791/2081 (2010).

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