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Analyzing tRNA Dynamics Using AQRNA-seq
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RNA Dizileme Hazırlığında AlkB Demetilaz ve Sonraki RNA-cDNA Hibrit Hidrolizi Kullanılarak RNA'larda Transkripsiyon Sonrası Metilasyon Giderimi
JoVE Journal
Genetics
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JoVE Journal Genetics
Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation
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RNA Dizileme Hazırlığında AlkB Demetilaz ve Sonraki RNA-cDNA Hibrit Hidrolizi Kullanılarak RNA'larda Transkripsiyon Sonrası Metilasyon Giderimi

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03:11 min

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February 02, 2024

DOI:

10.3791/201424-v

DOI:
10.3791/201424-v

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03:11 min
February 02, 2024

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2 Views
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1Department of Biological Engineering,Massachusetts Institute of Technology, 2Singapore-MIT Alliance for Research and Technology Antimicrobial Resistance Interdisciplinary Research Group

Tags

Post-transcriptional MethylationAlkB DemethylaseRNA Sequencing PreparationAlkB Reaction BufferRNA-cDNA Hybrid HydrolysisPhase SeparationPhenol-chloroform ExtractionGel ElectrophoresisEthidium Bromide StainingPCR Product VisualizationSodium Hydroxide NeutralizationAgarose Gel Preparation

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Post-Transcriptional Methylation Removal in RNAs Using AlkB Demethylase and Subsequent RNA-cDNA Hybrid Hydrolysis in RNA Sequencing Preparation. J. Vis. Exp. (Pending Publication), e201424, doi:10.3791/201424 (2024).

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