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Immunology and Infection

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病毒感染的人肠道类器官单层的实时成像

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Live Imaging of Virus-Infected Human Intestinal Organoid Monolayers

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病毒感染的人肠道类器官单层的实时成像

76 Views

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03:14 min

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January 19, 2024

DOI:

10.3791/201233-v

DOI:

10.3791/201233-v

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03:14 min

January 19, 2024

•

3 Views

J. Thomas Gebert, Francesca J. Scribano, Kristen A. Engevik, Joseph M. Hyser

¹Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology,Baylor College of Medicine

Tags

Live Imaging Virus-infected Human Intestinal Organoid Monolayers Epifluorescence Microscope Stage Top Incubator Bright Field Illumination Fluorescent Calcium Sensor Fluorescently Tagged Viruses Amino Fluorescent Staining Formaldehyde Ammonium Chloride Triton X-100 Blocking Solution Primary Antibodies Secondary Antibodies DAPI Rotavirus Infection HIO Monolayer

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