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Mouse Brain Histone Modification Quantification Using Flow Cytometry
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マウス脳ヒストン翻訳後修飾のフローサイトメトリーとデータ解析
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications
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マウス脳ヒストン翻訳後修飾のフローサイトメトリーとデータ解析

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02:23 min

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September 15, 2023

DOI:

10.3791/200982-v

DOI:
10.3791/200982-v

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02:23 min
September 15, 2023

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1Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health,University of British Columbia, 2Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health,University of British Columbia

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Flow CytometryMurine BrainHistone Post-translational ModificationsAntibody PanelCompensation MatrixRainbow Fluorescent BeadsPhotomultiplier Tube VoltageCompensation BeadsSide ScatterForward ScatterSinglet CellsFMOMicrogliaP2RY12Protein ExpressionMedian Fluorescence Intensity (MFI)LipopolysaccharideHistone 3 Lysine 27 AcetylationHistogram Analysis

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Flow Cytometry and Data Analysis of Murine Brain Histone Post-Translational Modifications. J. Vis. Exp. (Pending Publication), e200982, doi:10.3791/200982 (2023).

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