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Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

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Collection of Primordial Germ Cells from Drosophila Donor Embryos

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Collection of Primordial Germ Cells from Drosophila Donor Embryos

Collection of Primordial Germ Cells from Drosophila Donor Embryos

Cryopreservation of PGCs for Long-Term Preservation of Drosophila Strains

DOI:

10.3791/200839-v

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01:53 min

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December 01, 2023

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Kaori Nishimura*¹, Miho Asaoka*², Yurina Sakamaki*², Tatsuya Fukumoto*^3,4, Daisuke Tanaka, Satoru Kobayashi, Toshiyuki Takano-Shimizu-Kouno

¹KYOTO Drosophila Stock Center,Kyoto Institute of Technology, ²Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA),University of Tsukuba, ³Research Center of Genetic Resources,National Agriculture and Food Research Organization, ⁴Shizuoka Prefectural Ogasa High School

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Primordial Germ Cells PGCs Drosophila Embryos Micro Manipulator Forceps PGC Collection Glass Slide Transplantation Needle Somatic Cell Layer Silicone Oil Yolk Contaminants

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