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Journal
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Immunology and Infection
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جيل من 2D أحادي الطبقات من 3D البقري اللفائفي المعوي
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Generation of 2D Monolayers from 3D Bovine Ileal Enteroids
Please note that all translations are automatically generated.
Click here for the English version.
جيل من 2D أحادي الطبقات من 3D البقري اللفائفي المعوي
DOI:
10.3791/200825-v
•
04:32 min
•
April 05, 2024
•
Deborah Molehin
,
Jack Guinan
,
Brina Lopez
1
Department of Farm Animal Medicine, College of Veterinary Medicine
,
Midwestern University
Tags
2D Monolayers
3D Bovine Ileal Enteroids
Basement Membrane Extracellular Matrix
BME
Cell Culture
Cell Dissociation
Cell Suspension
Enteroid Fragments
Enteroid Growth Media
FBS Wash Media
Organoid Growth Media
TrypLE Express Enzyme
Trypan Blue Dye Exclusion
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توليد نظام استزراع أحادي الطبقة ثنائي الأبعاد مشتق من الإنتيرويد البقري للتطبيقات في البحوث الطبية الحيوية الانتقالية
جيل من 2D أحادي الطبقات من 3D البقري اللفائفي المعوي
القياس الكمي لسلامة الحاجز الظهاري والتقاء الطبقة الأحادية المشتقة من 2D البقري اللفائفي المشتق من اللفائفي
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