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Siembra de hepatocitos humanos primarios para establecer el sistema de cultivo hepático 2D+ totalmente humano
JoVE Journal
Biology
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JoVE Journal Biology
Plating Primary Human Hepatocytes for Setting Up the All-Human 2D+ Hepatic Culture System
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Siembra de hepatocitos humanos primarios para establecer el sistema de cultivo hepático 2D+ totalmente humano

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145 Views

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03:30 min

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October 20, 2023

DOI:

10.3791/200769-v

DOI:
10.3791/200769-v

•

03:30 min
October 20, 2023

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4 Views
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1Institute of Regenerative Medicine,LifeNet Health, 2Biologics Division,LifeNet Health, 3LifeSciences Division,LifeNet Health, 4Research and Development,LifeSciences, LifeNet Health

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Keyword Extraction:Primary Human HepatocytesPHHsFeeder CellsHepatocyte Thawing MediumBiological Safety CabinetHepatocyte SuspensionComplete Plating MediumCollagen-coated 24-well PlateComplete Culture Medium

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Odanga, J. J., Gianulis, E., Whaley, L., LeCluyse, E. L., Presnell, S., Weaver, J. R. Plating Primary Human Hepatocytes for Setting Up the All-Human 2D+ Hepatic Culture System. J. Vis. Exp. (Pending Publication), e200769, doi:10.3791/200769 (2023).

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