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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
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Aislamiento de núcleos individuales de tejidos de mamíferos congelados para la secuenciación de ARN de núcleos individuales
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing
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Aislamiento de núcleos individuales de tejidos de mamíferos congelados para la secuenciación de ARN de núcleos individuales

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346 Views

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02:02 min

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July 28, 2023

DOI:

10.3791/200321-v

DOI:
10.3791/200321-v

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02:02 min
July 28, 2023

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3 Views
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1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

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Keyword Extraction:Single NucleiSingle Nuclei RNA SequencingRobotic DissociatorNuclei Isolation CartridgeRNA InhibitorNuclei Extraction Run

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Stalder, L., Koechl, F., Hahn, K., Sultan, M., Prasad, M. K. Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing. J. Vis. Exp. (Pending Publication), e200321, doi:10.3791/200321 (2023).

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