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Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues
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Isolamento de Núcleos Únicos de Tecidos Congelados de Mamíferos para Sequenciamento de RNA de Núcleos Únicos
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing

Isolamento de Núcleos Únicos de Tecidos Congelados de Mamíferos para Sequenciamento de RNA de Núcleos Únicos

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02:02 min

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July 28, 2023

DOI:

10.3791/200321-v

DOI:
10.3791/200321-v

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02:02 min
July 28, 2023

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3 Views
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1Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

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Keyword Extraction:Single NucleiSingle Nuclei RNA SequencingRobotic DissociatorNuclei Isolation CartridgeRNA InhibitorNuclei Extraction Run

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Stalder, L., Koechl, F., Hahn, K., Sultan, M., Prasad, M. K. Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing. J. Vis. Exp. (Pending Publication), e200321, doi:10.3791/200321 (2023).

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