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Labeling and Imaging Cells in the Zebrafish Hindbrain
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Labeling and Imaging Cells in the Zebrafish Hindbrain
DOI:

09:17 min

July 25, 2010

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Chapters

  • 00:05Title
  • 00:53Introduction
  • 01:27Microinjection in the Zebrafish Hindbrain
  • 03:56Imaging mGFP-labeled Vibratome Sections
  • 06:26Live Imaging
  • 07:49Imaging Cells in the Hindbrain
  • 08:53Conclusion

Summary

Automatic Translation

Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.

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