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September 11, 2009
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Zebrafish cell transplantation enables the combination of genetics and embryology to generate tissue specific chimeras. This video demonstrates gastrula staged cell transplantations that have allowed our lab to investigate the roles of astroglial populations and specific guidance cues during commissure formation in the forebrain.
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Zebrafish GastrulaCell TransplantationDevelopmental BiologyCell FunctionsProtein MisexpressionForebrain CommissuresNeuron-glial Cell InteractionsAstroglial PopulationsGuidance CuesProjecting AxonsMidline CrossingZebrafish EmbryosEctopic Cell PlacementGene MisexpressionVital Dye TracerFluorescent Protein Expression
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Deschene, E. R., Barresi, M. J. Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation. J. Vis. Exp. (31), e1422, doi:10.3791/1422 (2009).
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