Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
感染性単核球症の小児からの末梢血サンプル中の免疫細胞亜集団の分離
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016,Chinese Academy of Medical Sciences
Bölümler
- 00:05Introduction
- 00:36Isolation of CD14+ Monocytes from PBMCs Using CD14 Microbeads
- 02:38Fluorescently Labeled Antibody Staining
- 03:52Flow Cytometry Parameter Setting
- 04:32Gating
- 06:18Cell Sorting and Collection via Flow Cytometry
- 07:00Results: Comparison of Immune Cell Subpopulations from Patients with Infectious Mononucleosis, Healthy Epstein Barr Virus Carriers, and Uninfected Samples
- 08:14Conclusion
