Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
イメージング質量分析 による 寒天上および感染組織内で増殖した細菌コロニーの微生物連携の検討
Bölümler
- 00:05Introduction
- 00:58Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
- 02:33MALDI Matrix Application via Robotic Spraying
- 03:12Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
- 04:37Imaging Mass Spectrometry Data Analysis and Compound Identification
- 05:57Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
- 07:45Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
- 08:08Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
- 09:09Conclusion
