Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
التحقيق في التعاون الميكروبي عن طريق التصوير تحليل قياس الطيف الكتلي للمستعمرات البكتيرية المزروعة على أجار وفي الأنسجة أثناء العدوى
Bölümler
- 00:05Introduction
- 00:58Ambient, Vacuum, and Heat-Mediated Drying of Clostridioides difficile and Enterococcus faecalis Bacterial Macrocolonies
- 02:33MALDI Matrix Application via Robotic Spraying
- 03:12Preparing Bacterial Macrocolonies for MALDI Imaging Mass Spectrometry Data Acquisition
- 04:37Imaging Mass Spectrometry Data Analysis and Compound Identification
- 05:57Cryosectioning of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues
- 07:45Preparation of Noninfected Control Versus Clostridioides difficile-Infected Mouse Cecal Tissues for Matrix Application and MALDI Imaging Mass Spectrometry
- 08:08Results: Imaging Mass Spectrometry Analysis of Bacterial Macrocolonies and Infected Murine Cecal Tissues
- 09:09Conclusion
