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Anjiyotensin II İndüklü Abdominal Aort Anevrizmasının Bir Fare Modelinde Santral Venöz Kateter ile İlaç Tedavisi ve 3D Ultrason ile İzleme
JoVE Journal
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JoVE Journal Tıp
Drug Treatment by Central Venous Catheter in a Mouse Model of Angiotensin II Induced Abdominal Aortic Aneurysm and Monitoring by 3D Ultrasound
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Anjiyotensin II İndüklü Abdominal Aort Anevrizmasının Bir Fare Modelinde Santral Venöz Kateter ile İlaç Tedavisi ve 3D Ultrason ile İzleme

2,719 Views

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10:09 min

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August 04, 2022

DOI:

10.3791/64124-v

DOI:
10.3791/64124-v

•

10:09 min
August 04, 2022

•

5 Views
, , , , , , , ,
1Division of Vascular Surgery, Department of General Surgery,Medical University of Vienna and Vienna General Hospital, 2Leeds Institute for Cardiovascular and Metabolic Medicine, School of Medicine,University of Leeds, 3Leeds Vascular Institute,Leeds General Infirmary, 4Department for Visceral, Thoracic and Vascular Surgery,Technical University of Dresden and University Hospital Carl-Gustav Carus, 5Division of Cardiovascular and Interventional Radiology, Division of Molecular and Gender Imaging, Department of Biomedical Imaging and Image Guided Therapy,Medical University of Vienna and Vienna General Hospital

Özet

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Bu protokol, apolipoprotein E (ApoE) eksikliği olan farelerde anjiyotensin II salınımı ile abdominal aort anevrizmasını indüklemek için ozmotik bir pompanın ve tekrarlanan ilaç tedavisi için juguler ven kateterli bir vasküler erişim portunun ardışık implantasyonunu tanımlar. Anevrizma gelişiminin 3D ultrason ile izlenmesi, dorsal implantlara rağmen etkili bir şekilde gerçekleştirilir.

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Mouse ModelAbdominal Aortic AneurysmDrug TreatmentCentral Venous Catheter3D UltrasoundJugular Vein CatheterizationAPOE DeficientAnesthesiaSurgical ProcedureVascular Access SystemCatheter PreparationDisinfectionBlunt DissectionExternal Jugular Vein

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Ibrahim, N., Klopf, J., Bleichert, S., Bailey, M. A., Busch, A., Stiglbauer-Tscholakoff, A., Eilenberg, W., Neumayer, C., Brostjan, C. Drug Treatment by Central Venous Catheter in a Mouse Model of Angiotensin II Induced Abdominal Aortic Aneurysm and Monitoring by 3D Ultrasound. J. Vis. Exp. (186), e64124, doi:10.3791/64124 (2022).

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