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一种同时分析人胰腺组织中N-糖肽和磷酸肽的自旋尖端富集策略
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Biyokimya
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JoVE Journal Biyokimya
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
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一种同时分析人胰腺组织中N-糖肽和磷酸肽的自旋尖端富集策略

2,083 Views

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09:16 min

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May 04, 2022

DOI:

10.3791/63735-v

DOI:
10.3791/63735-v

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09:16 min
May 04, 2022

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2 Views
, ,
1Kimya Bölümü,University of Wisconsin-Madison, 2School of Pharmacy,University of Wisconsin-Madison

Özet

Automatically generated

翻译后修饰（PTM）改变蛋白质结构和功能。同时富集多个PTM类型的方法可以最大限度地提高分析的覆盖率。我们提出了一个使用双功能Ti（IV）固定化金属亲和色谱法，然后进行质谱分析的方案，用于同时富集和分析胰腺组织中的蛋白质N-糖基化和磷酸化。

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Tabang, D. N., Wang, D., Li, L. A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues. J. Vis. Exp. (183), e63735, doi:10.3791/63735 (2022).

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