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体外模拟旁非规范Wnt信号传导

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Gelişim Biyolojisi

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JoVE Journal Gelişim Biyolojisi

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

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体外模拟旁非规范Wnt信号传导

DOI:

10.3791/63247-v

•

11:14 min

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December 10, 2021

•

Omar Toubat, Jongkyu Choi, S. Ram Kumar^2,3

¹Cerrahi Bölümü,Keck School of Medicine of USC, ²Pediatri Anabilim Dalı,Keck School of Medicine of USC, ³Heart Institute,Children’s Hospital Los Angeles

Bölümler

00:04Introduction
00:36Cell Plating in Co‐Culture System
02:50Wound Assay and Quantitative Assessment of Myoblast Migration
05:57Immunofluorescence Staining and Imaging of Migrating Myoblasts
08:29Results: Effects of Wnt5a and ROR2 Knockdown on Myoblast Migration and Morphology
10:28Conclusion

Özet

Otomatik Çeviri

本研究概述了一种高度可重复和易于处理的体外研究旁分泌非典型Wnt信号事件的方法。该协议用于评估旁分泌Wnt5a信号传导对小鼠神经嵴细胞和成肌细胞的影响。

Etiketler

Paracrine Non-canonical Wnt Signaling In Vitro C2C12 Cells O9-1 Cells SiRNA Knockdown Cell Culture Microscopy Imaging

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