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iPSC来源心肌细胞中的高通量光学控制和记录钙信号，用于毒性测试和表型药物筛选
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March 31, 2022
DOI:
1LumiSTAR Biotechnology, Inc., 2NEXEL Co., Ltd., 3Institute of Pharmacology, College of Medicine,National Taiwan University, 4Manchester Heart Centre, Manchester Royal Infirmary,Manchester University NHS Foundation Trust, 5Division of Cardiovascular Sciences, Manchester Academic Health Sciences Centre,University of Manchester, 6Division of Cell Matrix Biology and Regenerative Medicine,University of Manchester
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本协议描述了iPSC来源心肌细胞（iPSC-CMs）中触发细胞活性的全光学控制和观察，用于高通量药物筛选和毒性测试。显示了时间和空间上表型模式的多参数量化。证明了药物在数小时内的长期影响，或数天的顺序测量。
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Chang, Y., Su, W., Su, C., Chung, M., Chang, J., Li, Y., Kao, Y., Chen, W., Daniels, M. J. High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening. J. Vis. Exp. (181), e63175, doi:10.3791/63175 (2022).
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