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Cromatografía semidirigida de ultra alto rendimiento acoplada al análisis de espectrometría de masas de metabolitos fenólicos en plasma de adultos mayores
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April 22, 2022
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El objetivo de este protocolo es detectar metabolitos fenólicos en plasma utilizando un método de cromatografía semi-dirigida-espectrometría de masas.
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Semi-targetedUltra-high-performance ChromatographyMass SpectrometryPhenolic MetabolitesPlasmaElderly AdultsUPLC-MS/MSProtein PrecipitationMetabolite IdentificationBrosimum AlicastrumBioavailabilityBiotransformationUntargeted AnalysisSemi-targeted AnalysisMolecular FeaturesMass-to-charge RatioIsotopic DistributionFragmentation PatternPolyphenol Metabolites
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Muñoz-Bernal, Ó. A., Vazquez-Flores, A. A., Alvarez-Parrilla, E., Martínez-Ruiz, N. R., de la Rosa, L. A. Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults. J. Vis. Exp. (182), e63164, doi:10.3791/63164 (2022).
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