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老年人血浆中酚类代谢物的半靶向超高效色谱耦合质谱分析

JoVE Journal
Biyokimya
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JoVE Journal Biyokimya

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

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老年人血浆中酚类代谢物的半靶向超高效色谱耦合质谱分析

3,729 Views

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14:39 min

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April 22, 2022

DOI:

10.3791/63164-v

DOI:

10.3791/63164-v

•

14:39 min

April 22, 2022

•

14 Views

Óscar A. Muñoz-Bernal¹, Alma A. Vazquez-Flores¹, Emilio Alvarez-Parrilla¹, Nina R. Martínez-Ruiz¹, Laura A. de la Rosa¹

¹Department of Chemical Biological Sciences,Universidad Autónoma de Ciudad Juárez

Özet

该协议的目标是使用半靶向色谱 - 质谱法检测血浆中的酚类代谢物。

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Semi-targeted Ultra-high-performance Chromatography Mass Spectrometry Phenolic Metabolites Plasma Elderly Adults UPLC-MS/MS Protein Precipitation Metabolite Identification Brosimum Alicastrum Bioavailability Biotransformation Untargeted Analysis Semi-targeted Analysis Molecular Features Mass-to-charge Ratio Isotopic Distribution Fragmentation Pattern Polyphenol Metabolites

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