Dergi
/
Nörobilim
/
通过联合共聚焦和超分辨率显微镜表征小鼠的神经肌肉接头
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
Please note that all translations are automatically generated. Click here for the English version.

通过联合共聚焦和超分辨率显微镜表征小鼠的神经肌肉接头

DOI:
10.3791/63032-v

•

11:03 min

•

December 08, 2021

•

, , , , ,
1Genethon, 91000, Evry, France, 2Université Paris-Saclay, Univ Evry, Inserm, Genethon, Integrare research unit UMR_S951, 91000, Evry, France, 3Ecole Pratique des Hautes Etudes, PSL Research University, 75014, Paris, France

Bölümler

  • 00:04Introduction
  • 01:17Dissection of Muscles and Immunostaining
  • 03:21Image Acquisition
  • 03:58Image Analysis to Calculate Postsynaptic NMJ Endplate Volume, MIP Area, and Relative Tortuosity
  • 05:05Image Analysis to Quantify Presynaptic Neurofilament Accumulation and Synaptic Vesicle Glycoprotein 2 Staining
  • 06:04Calculating the Distance Between Acetylcholine Receptor (AChR) Stripes and Their Width
  • 08:22Results: Multi Parameter Postsynaptic NMJ Characterization, STED Imaging, and Morphometric Analysis of Presynaptic Axon Terminal Distribution
  • 10:09Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

该协议描述了一种通过组合共聚焦和STED显微镜对神经肌肉接头进行形态学分析的方法，该方法用于量化SMA和ColQ相关CMS小鼠模型中的病理变化。

Etiketler

Neuromuscular JunctionsConfocal MicroscopySuper-resolution MicroscopySTEDMuscle TeasingImmunostainingPresynaptic Axon TerminalsActive ZonesQuantitative Analysis3D StructureAnimal ModelsImage AcquisitionImage Analysis

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

神经元的密集核心囊泡的超分辨率成像

神经肌肉接头处：测量突触大小，碎片和改变突触蛋白密度利用共聚焦荧光显微镜

协议星形胶质细胞的三维共聚焦形态分析

为什么量化事项：表型的表征在<em>果蝇</em>幼虫神经肌肉接头

神经元电活动外记录与神经活性物质在清醒小鼠微电泳应用相结合

腓神经损伤方法：可靠检测，以确定和测试因素修复神经肌肉接头

触发细胞应激和死亡使用传统的紫外线激光共聚焦显微镜

腺相关病毒和共聚焦显微镜对器官脑切片胶质细胞器的可视化和实时成像

超分辨率成像研究原发神经元中蛋白质和突触标记的共同定位

单骨骼肌肉纤维解剖，用于全山神经肌肉结的免疫荧光和形态分析

Read Article