Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Immunocolorazione fluttuante del cervello di topo
1USDA/ARS Children’s Nutrition Research Center, Department of Pediatrics,Baylor Tıp Fakültesi, 2Department of Endocrinology, Union Hospital, Tongji Medical College,Huazhong University of Science and Technology, 3Hubei Provincial Clinical Research Center for Diabetes and Metabolic Disorder, 4Department of Molecular and Cellular Biology,Baylor Tıp Fakültesi
Bölümler
- 00:04Introduction
- 00:50Perfusion
- 02:57Cryosectioning
- 04:06Free-Floating Wisteria Florbunda Agglutinin Staining and Anti- Arginine Vasopressin Immunostaining
- 05:13Mounting
- 06:00Imaging
- 06:42Results: Double Immunofluorescent Staining of Free-Floating Mouse Brain Sections
- 07:21Conclusion
