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使用双光束扫描电子显微镜从玻璃体生物样品制备薄片进行冷冻电子断层扫描
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August 05, 2021
DOI:
1Centre for Ultrastructural Imaging,New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science,Birkbeck College, University of London, 3Electron Bio-Imaging Centre,Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory,Stanford University
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使用聚焦离子束铣削从冷冻生物样品中产生玻璃体网格片，用于冷冻电子断层扫描。
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Bisson, C., Hecksel, C. W., Gilchrist, J. B., Carbajal, M. A., Fleck, R. A. Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography. J. Vis. Exp. (174), e62350, doi:10.3791/62350 (2021).
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