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Microcrystal Electron Diffraction of Small Molecules
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JoVE Journal Biyokimya
Microcrystal Electron Diffraction of Small Molecules

Microcrystal Electron Diffraction of Small Molecules

DOI:
10.3791/62313-v

•

09:48 min

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March 15, 2021

•

,
1Howard Hughes Medical Institute,Kaliforniya Üniversitesi, Los Angeles, 2Department of Biological Chemistry,Kaliforniya Üniversitesi, Los Angeles, 3Fizyoloji Bölümü,Kaliforniya Üniversitesi, Los Angeles

Bölümler

  • 00:04Introduction
  • 00:34Preparing Small Molecule Samples and TEM Grids
  • 01:37Applying Sample to Grids by Creating a Homogenous Fine Powder
  • 02:51Freezing and Loading Grids into the TEM
  • 04:12Locating and Screening Nanocrystals
  • 07:33Collecting MicroED Data
  • 08:36Results: Analysis of MicroED Dataset from Carbamazepine Microcrystal
  • 09:10Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we describe the procedures developed in our laboratory for preparing powders of small molecule crystals for microcrystal electron diffraction (MicroED) experiments.

Etiketler

Microcrystal Electron DiffractionSmall MoleculesPowderNanometer Size CrystalsAtomic Resolution StructuresTEM GridsGlow DischargeFilter PaperPowder TransferLiquid Nitrogen

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