The signal-to-noise ratio of data is one of the most important considerations in performing X-ray diffraction measurements from microcrystals. The VMXm beamline provides a low-noise environment and microbeam for such experiments. Here, we describe sample preparation methods for mounting and cooling microcrystals for VMXm and other microfocus macromolecular crystallography beamlines.
Crawshaw, A. D., Beale, E. V., Warren, A. J., Stallwood, A., Duller, G., Trincao, J., Evans, G. A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline. J. Vis. Exp. (172), e62306, doi:10.3791/62306 (2021).