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单一肌纤维培养测定法用于评估体外成人肌肉干细胞功能
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
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单一肌纤维培养测定法用于评估体外成人肌肉干细胞功能

DOI:
10.3791/62257-v

•

09:19 min

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February 15, 2021

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, , , , ,
1Leibniz Institute on Aging, Fritz-Lipmann-Institute

Bölümler

  • 00:04Introduction
  • 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
  • 01:54EDL Muscle Isolation and Collagenase Digestion
  • 03:19Myofiber Dissociation and Culture
  • 04:20siRNA Transfection, Fixation, and IF Staining
  • 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
  • 08:34Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在该协议中，描述了肌肉干细胞的体外培养和功能分析方法，该方法保留了它们与其内源性生态位的大部分相互作用。

Etiketler

Muscle Stem CellsSingle Myofiber CultureEx VivoNicheSiRNA TransfectionPasteur PipettesHorse SerumEthanolTibialis AnteriorEDL MuscleDigestionIsolation MediumMyofibers

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