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血管灌注固定的穆林肺的空气膨胀
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JoVE Journal Nörobilim
Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation
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血管灌注固定的穆林肺的空气膨胀

DOI:
10.3791/62215-v

•

07:19 min

•

February 02, 2021

•

, , ,
1Division of Pulmonary, Sleep and Critical Care Medicine, Department of Medicine,National Jewish Health, 2Section of Pediatric Gastroenterology, Hepatology, and Nutrition,Children’s Hospital Colorado

Bölümler

  • 00:05Introduction
  • 00:43Air-Inflation Apparatus Preparation
  • 01:40Animal Preparation
  • 03:18Lung Air-Inflation, Perfusion, and Fixation
  • 04:42Lung Extraction
  • 05:42Results: Representative Lung Inflation, Extraction, and Histological Evaluation
  • 06:47Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

呈现是一种空气膨胀的方法，通过血管灌注固定肺部，保留细胞在气道、肺泡和间歇性中的位置，用于结构功能分析。恒定的气道压力通过空气膨胀室保持，而固定气道则通过右心室喷灌。肺被处理为组织学研究。

Etiketler

Air-inflationVascular Perfusion-fixationLung PreservationCell MorphologyAir Inflation ApparatusTracheaRight VentricleAbdominal WallDiaphragmThoracic CavitySternumTrachea IncisionLure Stub AdapterSuture

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