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监测人类痰样中的中性粒细胞和导管素 G 活性

JoVE Journal
İmmünoloji ve Enfeksiyon

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JoVE Journal İmmünoloji ve Enfeksiyon

Monitoring Neutrophil Elastase and Cathepsin G Activity in Human Sputum Samples

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监测人类痰样中的中性粒细胞和导管素 G 活性

14,247 Views

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09:23 min

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May 21, 2021

DOI:

10.3791/62193-v

DOI:

10.3791/62193-v

•

09:23 min

May 21, 2021

•

40 Views

Dario L. Frey*^1,2, Matteo Guerra*^1,2,3,4, Marcus A. Mall^1,2,5,6,7, Carsten Schultz^1,3,8

¹Translational Lung Research Center Heidelberg (TLRC),German Center for Lung Research (DZL), ²Dept. of Translational Pulmonology,University of Heidelberg, ³Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL),University of Heidelberg, ⁴Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University,University of Heidelberg, ⁵Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine,Charité – Universitätsmedizin Berlin, ⁶Berlin Institute of Health (BIH), ⁷German Center for Lung Research (DZL), Associated Partner Site, Berlin, ⁸Dept. of Chemical Physiology and Biochemistry,Oregon Health and Science University

Özet

本文所述的协议提供了一个指南，可视化和量化人类痰中中性粒细胞蛋白酶的活动。这种分析的应用范围从抗炎治疗的评估，到生物标志物验证、药物筛选和大队列临床研究。

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