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从多萨尔海马的急性切片准备全细胞记录和神经元重建在成人小鼠的登特陀螺
JoVE Journal
Nörobilim
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JoVE Journal Nörobilim
Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice
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从多萨尔海马的急性切片准备全细胞记录和神经元重建在成人小鼠的登特陀螺

DOI:
10.3791/61980-v

•

10:45 min

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April 03, 2021

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, ,
1Institute of Physiology,Christian-Albrechts-University Kiel

Bölümler

  • 00:00Introduction
  • 01:02Preparing the Bench for Slicing
  • 03:57Preparation of Dorsal Hippocampal Slices
  • 07:47Results: The Transversal Slice Shows Enhanced Viability Compared to the Coronal Slice
  • 10:10Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

我们提出了一个协议，准备从小鼠的单体中间海马的急性切片。我们将这种横向制剂与日冕切片在录音质量和记录神经元形态特征的保存方面进行比较。

Etiketler

Acute SlicesDorsal HippocampusWhole-cell RecordingNeuronal ReconstructionDentate GyrusAdult MiceBrain SlicingElektrofizyolojiVideo RecordingsTransversal SlicesProtecting SolutionVibratomeCutting SolutionTranscardial PerfusionIncubation ChamberSlice Storage Chamber

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