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使用纤维素凝块固定 的果蝇 组织用于实时成像

JoVE Journal
Gelişim Biyolojisi

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JoVE Journal Gelişim Biyolojisi

Applications of Immobilization of Drosophila Tissues with Fibrin Clots for Live Imaging

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使用纤维素凝块固定的果蝇组织用于实时成像

DOI:

10.3791/61954-v

•

08:52 min

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December 22, 2020

•

Jens Januschke, Nicolas Loyer

¹Cell & Developmental Biology, School of Life Sciences,University of Dundee

Bölümler

00:04Introduction
00:26Dissection of Drosophila Larval Brains
02:51Immobilization on Coverslip with Fibrin Clots
05:58Addition of Reagents during Live Imaging
07:00Results: Application of an ATP Analog on Immobilized Egg Chambers During Live Imaging
08:13Conclusion

Özet

Otomatik Çeviri

该协议描述了使用纤维蛋白块的样品固定应用，限制漂移，并允许在现场成像过程中添加和冲洗试剂。样品被转移到一滴含有培养基的纤维素中，然后通过添加血栓诱导聚合。

Etiketler

Immobilization Live Imaging Fibrin Clots Sample Preparation Larva Dissection Brain Dissection Culture Medium Fibrinogen Thrombin Forceps Pipette Borosilicate Glass Dish

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