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使用随机微种子基质筛选与 I3C 的蛋白质晶体的衍生
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Biyokimya
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JoVE Journal Biyokimya
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
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使用随机微种子基质筛选与 I3C 的蛋白质晶体的衍生

DOI:
10.3791/61894-v

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14:04 min

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January 16, 2021

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, , ,
1School of Biological Sciences,The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences,The University of Adelaide

Bölümler

  • 00:00Introduction
  • 01:51Preparation of 13C Stock
  • 02:24Addition of I3C to Protein Stock
  • 03:18Making a Rounded Probe
  • 04:04Making a Seed Stock
  • 06:33Setting Up an rMMS Screen
  • 08:08Data Processing
  • 11:21Representative Results
  • 13:22Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

本文提出了一种利用微种子在稀疏基质晶波中产生与 I3C（5-氨基-2，4，6-三叶磷酸）衍生的蛋白质晶体的方法。托盘可以使用液体点胶机器人或手动设置。

Etiketler

Protein CrystalDerivatizationI3CRandom Microseed Matrix ScreeningX-ray CrystallographyPhasing MoleculeAnomalous SignalSingle Wavelength Anomalous Discussion PhasingLithium I3CProtein CrystallizationStructure Solution

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