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下游免疫污染分析小鼠水母上皮周期特定阶段的转化-辅助解剖
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October 07, 2020
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1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit,University of Turku, Turku, Finland, 2Pediatri Anabilim Dalı,Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health,Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute,Monash University, Melbourne, VIC 3800, Australia
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该协议描述了代表半尖上皮周期特定阶段的成年小鼠半尼弗管段的透水辅助微分流，以及其中的细胞类型，以及壁球制剂和完整大肠管段的后续免疫污染。
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Mäkelä, J., Cisneros-Montalvo, S., Lehtiniemi, T., Olotu, O., La, H. M., Toppari, J., Hobbs, R. M., Parvinen, M., Kotaja, N. Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses. J. Vis. Exp. (164), e61800, doi:10.3791/61800 (2020).
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