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细菌性心内膜炎小鼠模型中感染参数的内注射与定量
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
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细菌性心内膜炎小鼠模型中感染参数的内注射与定量

DOI:
10.3791/61749-v

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07:24 min

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February 06, 2021

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, , , , ,
1Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology,Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

Bölümler

  • 00:04Introduction
  • 00:54Bacillus cereus Culture and Dilution
  • 01:48Mouse Intravitreal Injection
  • 04:02Eye Harvest
  • 04:43Intraocular Bacterial Count
  • 06:06Results: Representative Bacterial Endophthalmitis Mouse Model Validation
  • 06:47Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在这里，我们描述了一种细菌性心内膜炎小鼠模型中的内注射和随后的细菌定量方法。该协议可以扩展用于测量宿主免疫反应和细菌和宿主基因表达。

Etiketler

Intravitreal InjectionMouse ModelBacterial EndophthalmitisPathogenesisIntraocular InfectionQuantitationMicroorganismsHost Immune ResponsesGene ExpressionTreatment EffectivenessBiosafety Level TwoB. CereusMicroinjectorOphthalmic MicroscopeAnesthetized MouseReverse Action Forceps

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