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通过微透析对芯片上的蛋白质进行结晶，用于 原位 X射线衍射研究
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Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
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通过微透析对芯片上的蛋白质进行结晶，用于 原位 X射线衍射研究

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12:38 min

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April 11, 2021

DOI:

10.3791/61660-v

DOI:
10.3791/61660-v

•

12:38 min
April 11, 2021

•

23 Views
, , , , ,
1Université Grenoble Alpes, CEA, CNRS, IBS, 2CNRS, Solvay LOF, UMR 5258,Université Bordeaux, 3ELVESYS SAS

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本文详细介绍了采用透析方法和 原位 X射线衍射实验开发用于片上蛋白质结晶的微流体芯片的制造方案。微制造过程使得在芯片的两层之间将半透水再生纤维素透析膜与任何分子量切断相结合成为可能。

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Jaho, S., Junius, N., Borel, F., Sallaz-Damaz, Y., Salmon, J., Budayova-Spano, M. Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies. J. Vis. Exp. (170), e61660, doi:10.3791/61660 (2021).

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