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氢-铀交换质谱仪（HDX-MS）平台，用于研究肽生物合成酶
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11:32 min
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May 04, 2020
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在肽天然产品的生物合成过程中，兰西肽合成器催化多步反应。在这里，我们描述了一个连续的，自下而上的，氢-钚交换质谱仪（HDX-MS）的工作流程，可用于研究兰西肽合成器的构象动力学，以及肽天然产品生物合成中涉及的其他类似酶。
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Habibi, Y., Thibodeaux, C. J. A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes. J. Vis. Exp. (159), e61053, doi:10.3791/61053 (2020).
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