Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Ein Femtoliter Droplet Array für massive parallele Proteinsynthese aus einzelnen DNA-Molekülen
1SUGAR Program, X-star,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2Center for Mathematical Science and Advanced Technology,Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Deep-Sea Nanoscience Research Group,Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
Bölümler
- 00:04Introduction
- 00:33Cleaning and Silanizing the Glass
- 01:26Spin-Coating the Glass Substrate
- 02:40Photolithography, Etching, and Cleaning
- 03:47Fabricating the Microchamber Array Device
- 05:04Generating the Femtoliter Droplet Array (FemDA)
- 06:35Image Data Analysis
- 08:45Results: Cell-Free Protein Synthesis in FemDA
- 10:07Conclusion
