Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Diretto Assemblea di proteine Elastin-like in strutture supramolecolari definite e Incapsulamento di carico In Vitro
1Center for Biological Systems Analysis,University of Freiburg, 2Faculty of Biology,University of Freiburg, 3Freiburg Institute for Advanced Studies (FRIAS),University of Freiburg, 4BIOSS Centre for Biological Signalling Studies,University of Freiburg, 5IMTEK Department of Microsystems Engineering,University of Freiburg, 6Cluster of Excellence livMatS at FIT – Freiburg Center for Interactive Materials and Bioinspired Technologies,University of Freiburg
Bölümler
- 00:05Introduction
- 00:45Protein Expression and Preparation
- 03:38Dye-modification of Elastin-like Proteins via SPAAC
- 04:44THF Swelling Protocol
- 06:29BuOH Extrusion Protocol
- 07:19Results: THF Swelling Method and the BuOH Extrusion Method for the Guided Self-assembly of Amphiphilic ELPs into Supramolecular Structures
- 09:22Conclusion
All'interfaccia di solventi organici e acquosi, proteine arofhiliche elastine su misura si assemblano in complesse strutture supramolecolari come vescicoli, fibre e coacervate innescate da parametri ambientali. I protocolli di assemblaggio descritti producono compartimenti basati su membrana proteica (PMBC) con proprietà regolabili, consentendo l'incapsulamento di vari carichi.
