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共培养实验的奥里戈丁细胞和奥里戈丁酸基质的生成
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JoVE Journal Nörobilim
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
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共培养实验的奥里戈丁细胞和奥里戈丁酸基质的生成

DOI:
10.3791/60912-v

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09:05 min

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February 09, 2020

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, , , , ,
1Sorbonne Université, Inserm, CNRS, UMR7225,Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris,GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée

Bölümler

  • 00:04Introduction
  • 01:24Dissection
  • 02:23Tissue Dissociation
  • 04:13OL (Oligodendrocyte) Lineage Cells Harvesting and Culture
  • 06:30OCM (Oligodendrocyte-conditioned Medium) Production
  • 07:10Results: OL Lineage Cells Phenotype and Representative Applications
  • 08:20Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

本文展示了一种用于纯化寡核苷酸物和生产寡核苷酸调节介质的有效方法，可用于共培养实验。

Etiketler

OligodendrocytesOligodendrocyte-conditioned MediumCo-cultureMultiple SclerosisMyelinationCell CultureCerebral CorticesEnzymatic DigestionCell Filtration

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