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具有蓝色和红移通道性通道性神经元的长距离通道多普辛辅助电路映射
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Long-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins
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具有蓝色和红移通道性通道性神经元的长距离通道多普辛辅助电路映射

7,180 Views

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07:04 min

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February 07, 2020

DOI:

10.3791/60760-v

DOI:
10.3791/60760-v

•

07:04 min
February 07, 2020

•

3 Views
,
1Kresge Hearing Research Institute, Department of Otolaryngology – Head and Neck Surgery,University of Michigan

Özet

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通道多普辛辅助电路映射 （CRACM） 是一种精确的技术，用于在解剖学和/或基因识别的神经元组之间进行远程神经元投影的功能映射。在这里，我们描述了如何使用CRACM来映射听觉脑干连接，包括使用红移蛋白，ChrimsonR。

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Goyer, D., Roberts, M. T. Long-range Channelrhodopsin-assisted Circuit Mapping of Inferior Colliculus Neurons with Blue and Red-shifted Channelrhodopsins. J. Vis. Exp. (156), e60760, doi:10.3791/60760 (2020).

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