Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
Bölümler
- 00:04Introduction
- 01:02Methyl-Beta-Cyclodextrin (MBCD) Cholesterol-Enriching Solution Preparation
- 02:12Cholesterol-Saturated Tissue Enrichment
- 05:07Cholesterol Liposome-Enriched Phospholipid-Based Dispersion Preparation
- 06:06Xenopus Oocyte Cholesterol Enrichment
- 07:37Results: Representative Effects of Rat Cerebral Artery and Xenopus Oocyte Cholesterol Enrichment
- 09:22Conclusion
Two methods of cholesterol enrichment are presented: the application of cyclodextrin saturated with cholesterol to enrich mammalian tissues and cells, and the use of cholesterol-enriched phospholipid-based dispersions (liposomes) to enrich Xenopus oocytes. These methods are instrumental for determining the impact of elevated cholesterol levels in molecular, cellular, and organ function.
