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Generatie van Orthotopic alvleesklier tumoren en ex vivo karakterisering van tumor-Infiltrerende T cel cytotoxiciteit
JoVE Journal
Kanser Araştırmaları
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JoVE Journal Kanser Araştırmaları
Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity
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Generatie van Orthotopic alvleesklier tumoren en ex vivo karakterisering van tumor-Infiltrerende T cel cytotoxiciteit

12,059 Views

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06:16 min

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December 07, 2019

DOI:

10.3791/60622-v

DOI:
10.3791/60622-v

•

06:16 min
December 07, 2019

•

17 Views
, ,
1Division of Cancer, Department of Surgery and Cancer,Imperial College London, 2Centre for Cancer and Inflammation, Barts Cancer Institute,Queen Mary University of London, 3German Center for Neurodegenerative Diseases (DZNE)

Özet

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Dit protocol beschrijft de chirurgische generatie van orthotopic alvleesklier tumoren en de snelle vertering van vers geïsoleerde lymfatische alvleesklier tumoren. Na de spijsvertering kunnen levensvatbare immuuncelpopulaties worden gebruikt voor verdere downstream-analyse, inclusief ex-vivo stimulatie van T-cellen voor intracellulaire cytokine detectie door Flowcytometrie.

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Spear, S., McNeish, I. A., Capasso, M. Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity. J. Vis. Exp. (154), e60622, doi:10.3791/60622 (2019).

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