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使用 PRIMO 微模式系统对蛋白质的光诱导分子吸附，研究细胞对细胞外基质蛋白的反应
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October 11, 2019
DOI:
1Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research,The University of Manchester, 2School of Materials,The University of Manchester, 3Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester,Manchester Academic Health Science Centre, 4Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust,Manchester Academic Health Science Centre, 5Patoloji Bölümü,UMC Utrecht
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我们的总体目标是了解细胞如何感知细胞外线索，导致定向的斧头生长。在这里，我们描述了光诱导分子吸附蛋白质的方法，用于生成细胞外基质成分的定义的微模式，以研究控制斧头生长和路径发现的特定事件。
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Melero, C., Kolmogorova, A., Atherton, P., Derby, B., Reid, A., Jansen, K., Ballestrem, C. Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins. J. Vis. Exp. (152), e60092, doi:10.3791/60092 (2019).
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