Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in <em>Arabidopsis thaliana</em>

Melissa Bredow; Irina Sementchoukova; Kristen Siegel; Jacqueline Monaghan

doi:10.3791/59437

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Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

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Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

12,755 Views

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04:11 min

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May 21, 2019

DOI:

10.3791/59437-v

DOI:

10.3791/59437-v

•

04:11 min

May 21, 2019

•

16 Views

Melissa Bredow¹, Irina Sementchoukova¹, Kristen Siegel¹, Jacqueline Monaghan¹

¹Biyoloji Bölümü,Queen’s University

Özet

This paper describes two methods for quantifying defense responses in Arabidopsis thaliana following exposure to immune elicitors: the transient oxidative burst, and the inhibition of seedling growth.

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Arabidopsis Thaliana Pattern-triggered Oxidative Burst Seedling Growth Inhibition Immune Outputs Plant Genotypes Laboratory Equipment Leaf Discs Elicitors Reactive Oxygen Species Microplate Reader MS Medium Seedling Growth Growth Inhibition

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Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

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