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Profilage et identification de Nitropeptide illustrépar Angiotensin II
1Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 2Department of Biological Sciences,Notre Dame Üniversitesi, 3Department of Urology, Shanghai East Hospital,Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases,Notre Dame Üniversitesi, 5Harper Cancer Research Institute,Notre Dame Üniversitesi, 6Tumor Microenvironment and Metastasis Program,Indiana University Melvin and Bren Simon Cancer Center
Bölümler
- 00:04Başlık
- 00:32Nitro-Angiotensin II Desalting
- 01:30Primary Amine Alkylation by Dimethyl Labeling
- 02:37Nitrotyrosine to Aminotyrosine Reduction and Biotinylation, Enrichment, and Detection
- 06:03Results: Representative Mass Spectra Analyses
- 06:59Conclusion
Le profilage protéomique des protéines tyrosine-nitrated a été une technique provocante due à la basse abondance de la modification de 3-nitrotyrosine. Ici nous décrivons une approche nouvelle pour l'enrichissement et le profilage de nitropeptide en utilisant Angiotensin II comme modèle. Cette méthode peut être étendue à d'autres systèmes in vitro ou videodan vivo.
