Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Nitropeptide profilering en identificatie geïllustreerd door angiotensine II
1Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 2Department of Biological Sciences,Notre Dame Üniversitesi, 3Department of Urology, Shanghai East Hospital,Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases,Notre Dame Üniversitesi, 5Harper Cancer Research Institute,Notre Dame Üniversitesi, 6Tumor Microenvironment and Metastasis Program,Indiana University Melvin and Bren Simon Cancer Center
Bölümler
- 00:04Başlık
- 00:32Nitro-Angiotensin II Desalting
- 01:30Primary Amine Alkylation by Dimethyl Labeling
- 02:37Nitrotyrosine to Aminotyrosine Reduction and Biotinylation, Enrichment, and Detection
- 06:03Results: Representative Mass Spectra Analyses
- 06:59Conclusion
Proteomic profilering van tyrosine-nitraat eiwitten is een uitdagende techniek vanwege de geringe overvloed van de 3-nitrotyrosine modificatie. Hier beschrijven we een nieuwe aanpak voor nitropeptide verrijking en profilering met behulp van angiotensine II als model. Deze methode kan worden uitgebreid voor andere in vitro -of in vivo -systemen.
