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Isolamento e cultura de células-tronco neurais embrionárias de rato
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JoVE Journal Nörobilim
Isolation and Culture of Embryonic Mouse Neural Stem Cells

Isolamento e cultura de células-tronco neurais embrionárias de rato

17,069 Views

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09:04 min

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November 11, 2018

DOI:

10.3791/58874-v

DOI:
10.3791/58874-v

•

09:04 min
November 11, 2018

•

42 Views
, , , , , , ,
1Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology,ACECR, 2Department of Biology, Faculty of Basic Sciences,Islamic Azad University, 3Biyoloji Bölümü,ACECR Institute of Higher Education

Özet

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Aqui, apresentamos uma nova técnica microcirúrgica para o isolamento de células-tronco neurais da Eminência ganglionar embrião de rato E13 .

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Embryonic MouseNeural Stem CellIsolationCultureGanglionic EminenceMicro-dissectionSingle Cell SuspensionHEPES MEM BufferDissociationSuspension CultureNerve Cells

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Homayouni Moghadam, F., Sadeghi-Zadeh, M., Alizadeh-Shoorjestan, B., Dehghani-Varnamkhasti, R., Narimani, S., Darabi, L., Kiani Esfahani, A., Nasr Esfahani, M. H. Isolation and Culture of Embryonic Mouse Neural Stem Cells. J. Vis. Exp. (141), e58874, doi:10.3791/58874 (2018).

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