Dergi
/
Biyokimya
/
生物素和光裂解链接器所附 rna 对高分子配合物的单步纯化
JoVE Journal
Biyokimya
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Biyokimya
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
Please note that all translations are automatically generated. Click here for the English version.

生物素和光裂解链接器所附 rna 对高分子配合物的单步纯化

7,066 Views

•

08:12 min

•

January 03, 2019

DOI:

10.3791/58697-v

DOI:
10.3791/58697-v

•

08:12 min
January 03, 2019

•

3 Views
, ,
1Integrative Program for Biological and Genome Sciences,University of North Carolina at Chapel Hill, 2Department of Biochemistry and Biophysics,University of North Carolina at Chapel Hill

Özet

Automatically generated

采用肉毒分蛋白策略纯化的 rna* 蛋白复合物在变性条件下被洗脱, 以不适合进一步纯化和功能分析的形式进行溶液处理。在这里, 我们描述了这种策略的修改, 利用光裂解链接器在 rna 和温和的紫外线洗脱步骤, 产生本地和全功能的 rna 蛋白复合物。

Explore More Videos

RNA Protein ComplexesSingle-step PurificationBiotinPhoto-cleavable LinkerMass SpectrometryT7 Generated RNAAdaptor OligonucleotideMouse Nuclear ExtractEDTAStreptavidin Agarose BeadsBinding BufferCentrifugationTube Rotation

Read Article

Cite this Article

Skrajna, A., Yang, X., Dominski, Z. Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker. J. Vis. Exp. (143), e58697, doi:10.3791/58697 (2019).

Download .ris file

Copy

Share Video

.

Copy