Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Erfassung und Identifizierung von RNA-bindende Proteine mit Click-Chemie-gestützte RNA-Interaktom erfassen (CARIC) Strategie
1College of Chemistry and Molecular Engineering,Peking University, 2Beijing National Laboratory for Molecular Sciences,Peking University, 3Peking-Tsinghua Center for Life Sciences,Peking University, 4Synthetic and Functional Biomolecules Center,Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Peking University
Bölümler
- 00:04Başlık
- 00:38Preparation of Lysate of Metabolically Labeled and UV Cross-linked Cells
- 03:33Preparation of Samples for RNA-interactome Capture
- 05:13RNA-interactome Capture
- 07:45Results: Quality Control of Captured RNA-binding Proteins
- 08:53Conclusion
