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癌遺伝子が誘導する老化の中に活性酸素種および分泌老化関連の表現型正常ひと線維芽細胞の定量測定
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JoVE Journal Gelişim Biyolojisi
A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
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癌遺伝子が誘導する老化の中に活性酸素種および分泌老化関連の表現型正常ひと線維芽細胞の定量測定

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13:59 min

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August 12, 2018

DOI:

10.3791/57890-v

DOI:
10.3791/57890-v

•

13:59 min
August 12, 2018

•

3 Views
, ,
1Department of Biochemistry, College of Medicine,Dong-A University, 2Peripheral Neuropathy Research Center, College of Medicine,Dong-A University

Özet

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細胞内 ROS は、細胞老化の誘導に重要な役割を果たすに示されています。ここでは、細胞の老化にともなう ROS レベルを定量化するための敏感な試金について述べる。伝えられる様々 な加齢に伴う機能不全に貢献する老化関連分泌形質を評価するためのプロトコルをいたします。

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Kim, Y. Y., Um, J., Yun, J. A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence. J. Vis. Exp. (138), e57890, doi:10.3791/57890 (2018).

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